The invention discloses an application of thrombin aPC in drugs for prevention and treatment of diabetic cardiomyopathy induced by streptozotocin. The thrombin aPC can stabilize the expression of YB1by inhibiting ubiquitination of a cardiac muscle cell YB1 in a hyperglycemic state, so as to alleviate cardiomyocyte hypertrophy and fibrosis. The therapeutic study results indicate that the thrombinaPC has significant curative effects of alleviating cardiomyocyte hypertrophy and fibrosis, improving myocardial contractile functions and increasing ejection fraction.