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发明公开
CN107805619A 猪链球菌2型divIVA基因敲除突变株及其应用 无效 - 撤回

猪链球菌2型divIVA基因敲除突变株及其应用
摘要：
本发明属于基因工程领域，涉及猪链球菌2型divIVA基因敲除突变株及其应用。所述突变株ΔdivIV制备方法为：利用同源重组基因敲除技术将S.suis 2野生株05ZYH33染色体上编码DivIVA蛋白的divIVA基因进行基因敲除，经组合PCR产物电泳、RT-PCR和DNA测序证实，确定所获得的菌株为基因敲除突变株，命名为05ZYH33ΔdivIVA。用本发明所述突变株进行动物实验研究其致病性，其结果是对实验动物的毒力显著降低，可应用于研制猪链球菌2型减毒疫苗。
摘要（英）：

The invention belongs to the field of gene engineering, and relates to a Streptococcus suis type 2 divIVA gene knockout mutant strain and applications thereof. The mutant strain [delta]divIV preparation method comprises: carrying out gene knockout on the divIVA gene encoding DivIVA protein on the chromosome of a S.suis 2 wild strain 05ZYH33, wherein the PCR product electrophoresis, RT-PCR and DNAsequencing combined experiment results prove that the obtained strain is the gene knockout mutant strain, and is named 05ZYH33[delta]divIVA. According to the present invention, with the application of the obtained mutant strain to perform animal experiments so as to study the pathogenicity, the results show that the virulence on the experimental animal is significantly reduced, and the obtained mutant strain can be used for researching Streptococcus suis type 2 attenuated vaccines.

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