The invention discloses a winch hook tightening active safety protection system. A first oil port and a second oil port of a reversing valve are connected to a winch control oil circuit separately. The system further comprises a limiting switch arranged on a boom head of a telescopic boom; the limiting switch is in signal connection with a control valve and used for changing the conduction direction of a control valve; an oil feeding port of the control valve is connected with the second oil port of the reversing valve; an oil discharging port of the control valve is connected with a reset cavity of the reversing valve; and the limiting switch is pressed and triggered to send a control signal to the control valve, so that the control valve moves to a working station, the oil feeding port and the oil discharging port of the control valve are connected when the control valve is in the working station, and hydraulic oil enters the reset cavity so that the reversing valve can return to theinitial working station. When a lifting hook triggers the limiting switch, the control valve guides part of pressure oil to the reset cavity of the reversing valve, so that the reversing valve returns to the initial position, the ascending process of a winch is stopped, and collision between the lifting hook and the boom head of the mechanical arm is avoided. The invention further provides a crane comprising the winch hook tightening active safety protection system.