The invention discloses a detection method for a release rate of bFGF-entrapped hydrogel, wherein the detection method comprises the following steps: a) freeze-drying the bFGF-entrapped hydrogel, andcarrying out surface gold-spraying treatment; and soaking the bFGF-entrapped hydrogel in a PBS buffer solution; b) detecting the content of bFGF in a supernatant of the bFGF-entrapped SBMA hydrogel byan ELISA kit; c) preparing a plurality of ELISA protein standard substances by an ELISA protein solution and a diluent; d) taking a reaction plate, adding the ELISA protein standard substances and the bFGF-entrapped hydrogel solution into pores of the reaction plate, and repeatedly mixing; e) adding distilled water and a first antibody working fluid into each pore of the reaction plate, and repeatedly mixing; f) adding an enzyme-labeled antibody working fluid, and repeatedly mixing; g) adding a chromogenic substrate working fluid, and repeatedly mixing; h) adding a termination solution to stop the reaction; and i) carrying out absorbance detection of the content of the bFGF by a microplate reader, wherein the cumulative release amount of the bFGF is confirmed together by the contents in the step b and the step i. The detection method is relatively simple and is easy to operate, and the detection precision is high.