The invention provides a beta-lactan antibiotic grafted polymer and an application thereof. A method comprises the following steps: subjecting hydroxylmethyl polystyrene resin and diglycidyl ether tocondensation in the presence of a catalyst; subjecting an obtained product to a ring-opening reaction with amino on a 4-bit side chain of beta-lactan antibiotics, and carrying out repeated washing, soas to obtain the grafted polymer for detecting drug resistance of beta-lactan antibiotics; packing the polymer to a column, and monitoring peak appearance time of a sample in effluent by using an ultraviolet detector; and carrying out bromocresol purple detection on the packed column, thereby rapidly obtaining pharmaceutical sensitive information on the sample. The method is applicable to proteinsamples and is also applicable to the detection on the drug resistance of the beta-lactan antibiotics through bacterial living bodies and a thallus lysate, so that the beta-lactan antibiotic graftedpolymer can be applied to rapid drug resistance analysis in clinical laboratories and used for instructing doctors to determine clinical medication schemes as soon as possible.