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发明授权
CN107541499B 一种靶向免疫检测点TNFR2的CIK的制备及其应用 有权

一种靶向免疫检测点TNFR2的CIK的制备及其应用
摘要：
本发明公开了一种靶向免疫检测点TNFR2的CIK的制备，将CD8leader、人源化抗TNFR2的单链抗体、CD8的铰链区和跨膜区、CD3ζ胞内信号区依次串联构成，得到融合基因片段Leader‑scFv(TNFR2)‑CD8‑CD3ζ，将所述融合基因片段Leader‑scFv(TNFR2)‑CD8‑CD3ζ插入慢病毒表达载体，包装成携带Leader‑scFv(TNFR2)‑CD8‑CD3ζ编码基因的慢病毒，将所述携带Leader‑scFv(TNFR2)‑CD8‑CD3ζ编码基因的慢病毒感染患者自体淋巴细胞诱导的CIK，得到靶向免疫检测点TNFR2的CIK，该发明能够针对恶性肿瘤精准治疗。
摘要（英）：

The invention discloses preparation of CIK targeting immunodetection point TNFR2, comprising the following steps: CD8leader, humanized anti-TNFR2 single-chain antibody, hinge region and transmembraneregion of CD8 and CD3 Zeta intracellular signal region are successively connected in series to obtain a fusion gene fragment Leader-scFv(TNFR2)-CD8-CD3 Zeta; the fusion gene fragment Leader-scFv(TNFR2)-CD8-CD3 Zeta is inserted into a lentiviral expression vector to be packaged into lentivirus carrying Leader-scFv(TNFR2)-CD8-CD3 Zeta coding gene; and the lentivirus carrying the Leader-scFv(TNFR2)-CD8-CD3 Zeta coding gene infects a patient's autologous lymphocytes induced CIK so as to obtain CIK targeting the immunodetection point TNFR2. The CIK of the invention is used for accurate treatment ofmalignant tumor.

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