The invention discloses an automatic doffing and burner gauze reversal conveying mechanism. The mechanism comprises a sliding way component, a sliding way support and a push air cylinder; the sliding way component comprises a spindle conveying belt which is used for conveying and transporting a fully loaded spindle; the sliding way support is connected to the lower portion of the sliding way component and used for pushing separation of the spindle on a bobbin creel of a spinner, and the spindle is moved to the upper portion of the sliding way component and conveyed conveniently; meanwhile, the sliding way support is used for adjusting tightness of the spindle conveying belt; the push air cylinder is arranged between the sliding way support and the sliding way component and used for pushing the sliding way component to slide front and back, and accordingly the spindle conveying belt is in contact with the spindle. The automatic doffing and burner gauze reversal conveying mechanism is simple in structure, stable in running and low in cost, effectively lowers the manual and equipment manufacturing cost, and can be widely applied to various thread spinner equipment.