The invention discloses a method for arranging a channel of large-and-medium-sized mammals for a highway. The method comprises the steps of large-and-medium-sized mammal channel position selecting, type determining, scale determining, size selecting, inducing habitat arranging (vegetation design, water body design, terrain design and microhabitat design are included) and protection facility arranging (separation fence arranging, arranging of facilities capable of reducing noise and visual interference and taking of control measures capable of reducing man-made interference are included). According to the method for arranging the channel of the large-and-medium-sized mammals for the highway, arranging of the channel of the large-and-medium-sized mammals in newly-constructed or reconstructed and expanded highway engineering can be achieved, interference of highway engineering construction to large-and-medium-sized mammal moving and habitats is effectively reduced and avoided, and guarantee of transportation safety is facilitated.