The invention relates to a method for evaluating the effect of a compound on angiogenesis in a pathological state. According to the invention, a vascular endothelial growth factor (VEGF) is used for treating young zebra fish in a specific period to simulate a pathological state, then complete subintestinal peripheral vein length is used as an evaluation index, so the evaluation method for the effect of a drug on angiogenesis in the pathological state is successively established. The method has the advantages of simple operation, rapidness, stability, reliability and good repeatability, is more scientific and objective, presents the generation condition of spinous process and improves accuracy of results.