A micro-mixed chip based on fixed-potential induced charge electro-osmosis relates to the field of micro-mixed chips and enables the problem to be solved that the processing of an existing micro-mixed chip based on induced charge electro-osmosis has complex steps and is difficult to perform. Four exciting electrodes and two suspending electrodes are disposed on a glass substrate, and all the electrodes are membrane electrodes; a first flow path, a second flow path, a third flow path and a mixed flow path on the lower surface of a PDMS (polydimethylsiloxane) cover plate form a chip microchannel; the glass substrate is sealed to the PDMS cover plate, two sides of the mixed flow path are attached respectively to one ends of the first and second exciting electrodes and one ends of the third and fourth exciting electrodes, the end of the first exciting electrode faces the end of the third exciting electrode, and one end of the first suspending electrode is disposed in the middle between the above ends; the end of the second exciting electrode faces the end of the third exciting electrode, and one end of the second suspending electrode is disposed in the middle between the above ends. Potential differences between the two facing exciting electrodes are equal. The micro-mixed chip is applicable to mixing of micro-fluids.