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发明授权
CN106282147B 胱硫醚β-裂解修饰酶的制备方法及含胱硫醚β-裂解修饰酶试剂盒 有权

胱硫醚β-裂解修饰酶的制备方法及含胱硫醚β-裂解修饰酶试剂盒
摘要：
本发明提供了一种胱硫醚β‑裂解酶的修饰方法和含有该修饰酶的试剂盒。该方法包括如下步骤：S1：去除胱硫醚β‑裂解酶功能性氨基酸链中的Cys的氨基酸侧链的巯基，得到胱硫醚β‑裂解酶非功能性氨基酸链；S2：切除所述胱硫醚β‑裂解酶非功能性氨基酸链中的12个氨基酸，完成修饰。该试剂盒包括试剂R1和试剂R2。本发明可使试剂盒的开封稳定性达到35天，稳定性大大提高。
摘要（英）：

The invention provides a modification method of a cystathionine beta-cracking modification enzyme and a kit containing the cystathionine beta-cracking modification enzyme. The method comprises the following steps of S1, removing hydrosulphonyls of Cys amino acid side chains in cystathionine beta-cracking enzyme functional amino acid side chains to obtain cystathionine beta-cracking enzyme non-functional amino acid side chains; S2, cutting away 12 amino acids in the cystathionine beta-cracking enzyme non-functional amino acid side chains to complete the modification. The kit comprises a reagent R1 and a reagent R2. The unsealing stability of the kit can reach 35 days; the stability is greatly improved.

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