The invention discloses an oocyte aggregation device. The oocyte aggregation device comprises an aggregation ring (1), an aggregation ring handle (2), a lock spring clamp (3), a plastic clamp ring (4) and an oocyte pushing needle (5); the aggregation ring (1) and the aggregation ring handle (2) are of an integral structure, the aggregation ring (1) is fixed into a ring shape through the lock spring clamp (3), the size of the aggregation ring (1) is adjustable, and the oocyte pushing needle (5) is connected with the aggregation ring handle (2) through the plastic clamp ring (4). By adopting the oocyte aggregation device, an appropriate quantity of free oocytes which are uniformly distributed can be quickly and conveniently aggregated on the condition that the shape structure of the oocytes is not influenced when the oocytes are shot by an anatomical lens or a microscope, therefore, images of the appropriate quantity of free oocytes which are uniformly distributed can be shot in a single image, oocyte image collection is quickly completed, and experimental cost and capital cost are reduced.