The invention provides an ajoene microcapsule powder product and its preparation method and use. The ajoene microcapsule powder product can be processed to form a plurality of dosage forms so that an application range is widened and properties and stability are good. The preparation method comprises carrying out extraction on garlicin-containing raw materials through water, filtering the extract to obtain a garlicin aqueous solution, extracting garlicin in the garlicin aqueous solution through an organic solvent, collecting the organic solvent phase, removing water, drying the garlicin to obtain garlicin-containing garlic oil, preparing a beta-cyclodextrin aqueous solution, embedding the garlicin-containing garlic oil with the beta-cyclodextrin aqueous solution to obtain a beta-cyclodextrin suspension, drying and crushing the beta-cyclodextrin suspension, and sieving the powder to obtain the ajoene microcapsule powder product. The preparation method can be used for ajoene microcapsule powder product preparation.