The invention discloses a transfer method of a hexagonal boron nitride film. A two-dimensional (2D) atom layer material prepared by a traditional chemical vapor deposition method (CVD) is generally grown on the surface of a metal catalyst; then, polymethyl?methacrylate (PMMA) is used as an aid for transferring the 2D material onto a target substrate; and finally, organic solvent is used for removing PMMA. But, the organic solvent is difficult to totally remove PMMA, one part of PMMA is remained on the surface of the 2D material to cause pollution; and PMMA cannot be totally removed even if through high-temperature oxidation or atmospheric reduction. A middle layer is introduced between the transferred 2D material hexagonal boron nitride and PMMA, so that the surface pollution of the hexagonal boron nitride from PMMA is prevented when realizing transfer of the hexagonal boron nitride onto the substrate.