The invention provides a pulse waveform testing circuit. The circuit comprises a pulse collection module, a pulse width detection module and a pulse capturing module, wherein the pulse collection module collects single-particle transient pulses generated by single-particle bombardment; the pulse width detection module is connected with the pulse collection module, detects the pulse widths of the single-particle transient pulses in different voltage values, and generates multiple corresponding pulse signals; and the pulse capturing module is connected with the pulse width detection module, and converts the pulse widths which are detected in different voltage values into digital signals. The pulse waveform testing circuit can be used to measure the real waveform of single-particle effect, and further establish a more accurate transient current pulse model of the single-particle effect, and the testing circuit has significance in reinforced design for anti-radiation circuits.