The invention discloses an LED driving chip and an over-temperature protection point adjusting unit thereof. The over-temperature protection point adjusting unit comprises an operational amplifier module, a current mirror module, a variable-resistance unit and a current amplification module, wherein the operational amplifier module is used for providing loading voltage and guarantees that stable voltage is provided when multiple loads exist or a single load is larger; the current mirror module is used for providing mirror current for current output by the operational amplifier module; the current amplification module is used for amplifying the mirror current according to default multiple; the variable-resistance unit is used for setting the over-temperature protection point size. The LED driving chip and the over-temperature protection point adjusting unit thereof have the benefits as follows: the LED driving chip is effectively prevented from being burnt out due to overhigh working temperature, meanwhile, the continuous working time of LED lighting equipment is prolonged as far as possible, and convenience is brought to a user.