The invention discloses a preparation method of morehella esculenta nutrition powder. The morehella esculenta nutrition powder is prepared by taking morehella esculenta as a raw material for the first time. The nutrition powder is simple in process, contains rich unsaturated fatty acids, various human essential amino acids and crude fibers, and is an edible fungus processed product integrating functions of nutrition, nourishing and regulation of the human immune function. The preparation method is simple in process, low in cost, capable of realizing mechanized production and easy for industrial popularization; meanwhile, the value of the morehella esculenta is further developed, development of morehella esculenta research and application is promoted powerfully, and good social and economic benefits are realized.