The invention discloses an imitative cheese in slice and a preparation method of the imitative cheese in slice. The imitative cheese in slice is prepared from the following raw materials: 30-60% of skimmed milk, 10-35% of milk fat, 0.5-1.5% of emulsifying salt, 0.5-2% of an acidity regulator, 2-5% of table salt and the balance being water. The preparation method comprises the following steps: (1) sterilizing the skimmed milk and performing ultrafiltration concentration at 30-50 DEG C by 1.3-7 times; (2) performing spray-drying to obtain a milk protein concentrate; (3) dissolving at 32-60 DEG C so that the content of solids can be within the range of 20-40%, regulating the pH to the range of 4.8-5.5 and adding the table salt; (4) adding the milk fat and the emulsifying salt, heating to the range of 45-60 DEG C and then homogenizing; and (5) heating to the range of 93-97 DEG C, preserving heat for 1-3 minutes, stirring, cooling and then packaging in a slice packaging machine. The preparation method of the imitative cheese in slice breaks through the limitation that a natural cheese is taken as a raw material in an existing imitative cheese technology, and is high in process continuation degree; the product is relatively good in taste and flavor; the production cost is reduced.