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发明公开
CN104560864A 利用CRISPR-Cas9系统构建的敲除IFN-β基因的293T细胞系 失效 - 权利终止

利用CRISPR-Cas9系统构建的敲除IFN-β基因的293T细胞系
摘要：
本发明公开了利用CRISPR-Cas9系统构建的敲除IFN-β基因的293T细胞系。本发明所提供的敲除IFN-β基因的293T细胞系，具体为人胚肾细胞293T-KO-IFN-β，它在中国微生物菌种保藏管理委员会普通微生物中心的保藏编号为CGMCC No.10096。实验证明，本发明所得的敲除IFN-β基因的293T细胞系不能正确的表达IFN-β蛋白。由于IFN-β蛋白具有抗病毒作用，故B型流感病毒在野生型的293T细胞中不能大量的增殖。而敲除IFN-β基因的293T细胞系由于不能正确的表达IFN-β蛋白，故可用于B型流感病毒在该细胞系中更好的增殖，获得更大量病毒用于实验研究。
摘要（英）：

The invention discloses an IFN-beta gene knocked out 293T cell line built by utilizing a CRISPR-Ca9 system. The IFN-beta gene knocked out 293T cell line disclosed by the invention is specifically a human embryo kidney cell 293T-KO-IFN-beta, and the preservation number thereof in the Common Microbe Preservation Center of China Microbe Bacterial Preservation Administration Committee is CGMCC No.10096. Experiments prove that the IFN-beta gene knocked out 293T cell line disclosed by the invention cannot be used for correctly expressing IFN-beta protein. The IFN-beta protein has an anti-virus effect, so influenza virus B cannot be greatly multiplied in wild type 293T cells. The IFN-beta gene knocked out 293T cell line cannot be used for correctly expressing IFN-beta protein, so that the influenza virus B can be multiplied better in the cell line, and more viruses can be obtained for experiment researches.

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