The invention discloses a tobacco flavor prepared from liquorice fermentation and application of the tobacco flavor. A dominant microorganism is separated from fresh tobacco leaves of flue-cured tobacco, and the classification name of the dominant microorganism is Lodderomyces elongisporus. CGMCC 6645; the Lodderomyces elongisporus is inoculated into a culture medium, taking liquorice as a raw material, for fermentation cultivation; a fermentation product after culture has a natural fragrance, and contains various aroma components, such as 2,4-resacetophenone, 2,4,5-methylphenol, 3,4-dimethyl benzyl alcohol and phenethyl alcohol. The tobacco flavor is added to tobacco shreds, and thus, the aroma and the moistening effect of a cigarette can be enhanced, and the cigarette quality is improved; the sweetness of smoke is rich, local offensive odor and wood odor are reduced, the throat comfort is enhanced, the remaining taste is comparatively comfortable, and the mouth astringency is strong; the whole production process for the tobacco flavor is simple and practical, and the tobacco flavor has a good application prospect.