The invention relates to a detection kit, and in particular relates to a detection kit for the content of delta-amino-gamma-aminolaevulini acid (ALA), which belongs to the field of biomedicine. The detection kit comprises five components namely a reagent A, a reagent B, a reagent C, a reagent D and a regent E. The detection kit for detecting the content of ALA in a tissue is formed on the basis of a transgenic mouse model of acute prophyries, namely an ALASE over-expression transgenic mouse model; furthermore, the method is easy and convenient to implement and has repeatability and applicability.