The invention relates to logistics equipment, in particular to a combination and separation device of a cigarette packing box. The device is installed and applied in a sorting system of the cigarette packing box and is formed by sequentially connecting a reversing channel, an accumulating channel, a cigarette box steering channel, an aligning channel and a cigarette tidying channel. The device is used in combination with other devices for use. The other devices move a cigarette packing box group from a tray to the device, the device separates the cigarette packing box group into single cigarette boxes with the same putting order, and the separation task is completed. The device combines the single cigarette boxes conveyed by a conveying system into the cigarette packing box group according to a specified putting order, the other devices move the cigarette packing box group onto the tray, and the combination task is completed. The device has the advantages of reasonable structure, convenience in operation, stable running and high working efficiency, and the labor intensity and the running cost are greatly reduced.