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发明公开
CN103194465A 5-烯醇丙酮莽草酸-3-磷酸合酶基因的分离 失效 - 权利终止

5-烯醇丙酮莽草酸-3-磷酸合酶基因的分离
摘要：
本发明属于基因工程技术领域，具体涉及一种抗草甘膦基因5-烯醇丙酮莽草酸-3-磷酸合酶(EPSPS)基因的分离鉴定，所述的5-烯醇丙酮莽草酸-3-磷酸合酶(EPSPS)基因的核苷酸序列如SEQ ID NO：1所述，该基因编码的氨基酸序列如SEQ ID NO：2所述。5-烯醇丙酮莽草酸-3-磷酸合酶(EPSPS)基因是从深海细菌Janibacter limosus菌株的基因组文库中克隆得到，包含该基因质粒的重组大肠杆菌LZD001保藏在中国典型培养物保藏中心，其保藏编号为CCTCC NO：M2011493。经过生物学实验验证，证明该基因对草甘膦具有一定的耐受性。
摘要（英）：

The invention belongs to the technical field of gene engineering, and in particular relates to isolation and identification of an anti-glyphosate gene 5-enol acetone shikimic acid-3-phosphate synthase (EPSPS). The EPSPS gene has a nucleotide sequence as described in a SEQ ID NO:1, and an amino acid sequence encoding the gene is shown as a SEQ ID NO:2. The EPSPS gene is cloned from a genomic library of a deep-sea bacterium Janibacter limosus strain. A recombinant Escherichia coli LZD001 containing the plasmid of the gene is preserved in China Center for Type Culture Collection, and has a preservation number of CCTCC No:M2011493. Biological experiments prove that the gene has certain tolerance to glyphosate.

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