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发明公开
CN103172712A 严重急性呼吸道综合征SARS冠状病毒非结构蛋白nsp2的羧基端区域的表达纯化及非结构蛋白nsp2的羧基端区域的晶体结构 无效 - 撤回

严重急性呼吸道综合征SARS冠状病毒非结构蛋白nsp2的羧基端区域的表达纯化及非结构蛋白nsp2的羧基端区域的晶体结构
摘要：
本发明提供一种严重急性呼吸道综合征SARS冠状病毒非结构蛋白nsp2的羧基端区域的晶体三维结构，其中SARS冠状病毒非结构蛋白nsp2的氨基端区域为所述SARS冠状病毒非结构蛋白nsp2的从约110～约150位氨基酸至在约600至638位范围内的氨基酸，其中所述晶体三维结构中的原子具有表1中所列的至少40％的原子坐标，或者SARS冠状病毒非结构蛋白nsp2的氨基端的晶体三维结构中至少40％氨基酸的主链碳骨架的原子结构坐标与表1中的坐标的平均根方差小于或等于1.5埃。本发明还提供对SARS冠状病毒非结构蛋白nsp2的表达、纯化和结晶的方法；以及SARS冠状病毒非结构蛋白nsp2的晶体结构及晶体结构在功能研究、潜在药物筛选及药物设计方面的理论指导意义和应用。
摘要（英）：

The invention provides a crystalline three-dimensional structure of a carboxyl terminal region of an SARS coronavirus non-structural protein nsp2. The amino terminal region of the SARS coronavirus non-structural protein nsp2 comprises amino acids having positions in a range of about 110-150 to about 600-638 of the SARS coronavirus non-structural protein nsp2; and atoms in the crystalline three-dimensional structure have at least 40% of atomic coordinates in a table 1, or the average root mean square deviation of the main-chain carbon-skeleton atomic structure coordinates of the at least 40% amino acids of the crystalline three-dimensional structure of the amino terminal region of the SARS coronavirus non-structural protein nsp2 and the coordinates in the table 1 is not greater than 1.5 angstroms. The invention also provides an expression, purification and crystallization method of the SARS coronavirus non-structural protein nsp2, the crystalline structure of the SARS coronavirus non-structural protein nsp2, and theoretic guiding meanings and applications of the crystalline structure in functional researches, latent drug screening and drug designs.

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