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发明公开
CN102964435A 截短型溶血链球菌溶菌素O及利用其的检测试剂盒 有权 转让

截短型溶血链球菌溶菌素O及利用其的检测试剂盒
摘要：
本发明提供一种截短型溶血链球菌溶菌素O基因与ASO检测试剂盒。本发明人克隆部分SLO基因片段，将该部分的SLO基因克隆到大肠杆菌等表达载体中，使之产量表达并利于纯化。具体而言，本发明人提供一种DNA序列，其碱基序列如SEQ ID NO：1所示。根据本发明，截短的SLO序列能使SLO蛋白的产量大大提高，每升细菌培养物能纯化得到150mg以上的SLO蛋白，纯度90％以上，经过免疫学实验证明，这一分段表达的SLO依然保持了特异的免疫原性。
摘要（英）：

The invention provides a truncated-form streptococcus hemolyticus bacteriolysin O gene and an ASO detection kit. According to the invention, part of SLO gene fragments are cloned, and the part of SLO genes are cloned into an expression vector of escherichia coli and the like so as to realize mass expression and facilitate purification. In particular, the invention provides a DNA sequence which has a base sequence as shown in SEQ ID NO:1. According to the invention, the truncated-form SLO sequence can greatly improve the yield of SLO protein; more than 150 mg of SLO protein can be obtained through purification of one liter of bacterial culture, and the purity is more than 90%; immunological tests prove that the sectionally expressed SLO still maintains the specific immunogenicity.

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