The invention discloses polyphenol oxidase expressed by laticifers of a rubber tree, an encoding gene of the polyphenol oxidase and application of the polyphenol oxidase and the encoding gene. The polyphenol oxidase can be expressed in laticifer cells of the rubber tree, has a sequence which is shown as SEQ ID NO:1 in a sequence table, and is named HbPPO1; and the HbPPO1 encodes 600 amino acids and has a sequence which is shown as SEQ ID NO:2 in the sequence table, the molecular weight of the HbPPO1 is about 68kDa, the isoelectric point is about 8.45, the charge number is about 7.96, and the HbPPO1 is basic protein. The enzyme or derived protein of the enzyme at least can be used for bleaching or oxidizing colored substances in food and industry fields; the antisense expression of the partial or entire sequence of the gene at least can inhibit a browning phenomenon in the tissue culture of rubber trees and prolong the latex drainage time of the rubber trees, so that the yield of rubber is improved; and the over-expression of the gene or a functional derived gene which is obtained by substituting and/or inserting and/or deleting one or more base pairs at least can improve the disease and pest resistance of the rubber trees.