The invention relates to a Honglong antalgic medicinal composition, and a preparation method and application thereof. The composition is prepared from the following crude medicaments in parts by weight: 40 to 55 parts of ethanol extract, 25 to 45 parts of aqueous extract and 12 to 28 parts of fine medicinal material powder. According to the novel extraction preparation method, the medicinal materials in the formula are separately extracted by different solvents, and mineral medicaments and toxic medicinal materials in the formula are decocted for a long time, so that effective components can be fully extracted from the mineral medicaments, and the toxicity of the toxic medicinal materials is lowered. On the premise of reserving the curative effects of the conventional preparation, a medicinal preparation prepared by the method can be fast absorbed, and is fast in effect and high in bioavailability, and active ingredients can reach a focus part in a centralized way. In addition, compared with the conventional Honglong antalgic tablet preparation, the preparation prepared from the composition has better curative effects.