The invention relates to a multi-epitope artificial antigen of plasmodium falciparum and an application thereof. The invention designs a polynucleotide with a sequence as shown in SEQ ID No:1 and a polypeptide with a sequence as shown in SEQ ID No:2, wherein the polynucleotide and the polypeptide are used as artificial antigens to prepare vaccines, and antibodies can be obtained from the antigens and are used to prepare immune preparations against plasmodium falciparum infections and kits for detecting malaria infections. Compared with anti plasmodium falciparum vaccines in the prior art, the multi-epitope artificial antigen of the invention has high immunogenicity and antigen recognition diversity, and the antibody of the invention has high inhibition efficiency on the in vitro growth of plasmodium falciparum.