The invention provides a head-body separation device with a guide function, comprising a guide assembly and a thrust assembly, wherein the guide assembly and the thrust assembly are arranged in a cylinder body and respectively comprise a guide push rod and a thrust spring which are arranged in the cylinder body; the front end of the guide push rod extends out of a front cover of the cylinder bodyand is used as a thrust end pushing against the head, namely the rear end of an aircraft, a piston which is matched with the inner diameter of the cylinder body is arranged at the rear end of the guide push rod, a compression spring which is arranged in a cylinder body chamber is arranged between the piston and a rear cover of the cylinder body, and the rear cover of the cylinder is fixed on the inner wall of a separation body through a base. The maximum stroke L of the guide push rod is smaller than the maximum compression amount 5mm-10mm of the spring. The maximum stroke L of the guide pushrod is greater than the maximum length H about 5mm-10mm of a protruding structure on the rear end surface of the aircraft. The front end of the guide push rod is sunk in a groove at the rear end of the aircraft.