The invention discloses a colloidal gold test strip for detecting antibodies against akabane virus and a preparation method thereof. The test strip comprises a bottom lining, and an absorbent pad, a nitrocellulose membrane, a glass fiber membrane and a sample mat are arranged on the bottom lining; a detection belt and a quality-control belt are arranged on the nitrocellulose membrane; and G1 recombinant proteins are coated in the detection belt, the antibodies against the akabane virus are coated in the quality-control belt, and colloidal gold-G1 recombinant proteins are coated in the glass fiber membrane, wherein the amino acid sequence of the G1 recombinant protein is shown in a sequence table SEQ ID NO.2. The colloidal gold test strip of the invention has the advantages of simple, efficient, peculiar and sensitive detection, low cost, and the like. The method for preparing the colloidal gold test strip comprises the steps of preparing RNA; preparing the G1 recombinant proteins; preparing the colloidal gold-G1 recombinant proteins; and preparing the antibodies against akabane and preparing the colloidal gold test strip. By using the preparation method, the colloidal gold test strip with good specificity and high sensitivity can be obtained, and meanwhile, the preparation method is simple, and low in cost.