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发明公开
CN101914569A 培育转葡激酶基因番茄的方法 无效 - 驳回

培育转葡激酶基因番茄的方法
摘要：
本发明公开了一种培育转葡激酶基因番茄的方法，该方法包括以下步骤：(1)构建含有葡激酶编码基因的重组植物表达载体；(2)用构建的重组植物表达载体转化番茄植株，得到转葡激酶基因番茄。本发明成功获得了转SAK基因的番茄植株，转基因植株表达的SAK蛋白经溶圈法证明具有溶栓活性，为以番茄为生物反应器大规模生产葡激酶奠定了基础。
摘要（英）：

The invention discloses a method for culturing a staphylokinase (SAK) transgenic tomato, comprising the following steps: (1) building a recombinant plant expression vector containing an SAK encoding gene; and (2) converting a tomato plant into the SAK transgenic tomato by the built recombinant plant expression vector. The method of the invention can help successfully obtain the SAK transgenic tomato plant; and a solusphere method proves that SAK protein expressed by the transgenic plant has thrombolytic activity, thus laying the foundation for large-scale production of SAK by taking the tomato as a bioreactor.

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