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发明授权
CN101899511B 应用AS-PCR检测牛白细胞粘附缺陷的方法 失效 - 权利终止

应用AS-PCR检测牛白细胞粘附缺陷的方法
摘要：
本发明属于家畜分子检测技术领域，具体涉及应用AS-PCR方法在体外检测牛白细胞粘附缺陷的方法。制备了一种用于牛粘附缺陷检测方法的特异性PCR引物，它包括：CD18基因扩增引物：突变型扩增产物长度为500bp；引物1：5’AAGATGACGAGGAGCAGAA 3’，引物2：5’GTCCATCAGGTAGTACAGGC 3’；野生型扩增产物长度为818bp；引物3：5’CAAGGGCTACCCCATCGA 3’，引物4：5’CAGGACTGCGTGGCTAAA 3’。本发明制备的牛白细胞粘附缺陷的方法的特异性引物，可直接应用于在体外开展牛粘附缺陷的致病基因的筛选，以利于对牛粘附缺陷个体进行早期淘汰。
摘要（英）：

The invention belongs to the technical field of livestock molecular detection, in particular relates to a method for detecting bovine leukocyte adhesion deficiency by applying an allele specific polymerase chain reaction (AS-PCR). A specific PCR primer used by a method for detecting bovine adhesion deficiency is prepared and comprises a CD18 gene amplification primer, wherein the length of a mutant amplification product is 500bp, a primer 1 is 5'AAGATGACGAGGAGCAGAA 3', and a primer 2 is 5'GTCCATCAGGTAGTACAGGC 3'; and the length of a wild amplification product is 818bp, a primer 3 is 5'CAAGGGCTACCCCATCGA 3', and a primer 4 is 5'CAGGACTGCGTGGCTAAA 3'. The specific primer used by the method for detecting the bovine leukocyte adhesion deficiency can be directly applied to in-vitro screening of disease-causing genes of the bovine adhesion deficiency so as to contribute to the early elimination of an individual with the bovine adhesion deficiency.

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