The invention discloses a porcine hemagglutinating encepha lomyelitis virus binding protein and a preparation method thereof. A protein bound with the porcine hemagglutinating encepha lomyelitis virus is prepared by a method of establishing a phage library. The binding protein has the effect of blocking the porcine hemagglutinating encepha lomyelitis virus from infecting nerve cells so as to achieve the antiviral effect. The targets are pertinent, so the porcine hemagglutinating encepha lomyelitis virus binding protein can solve the problems of ineffectiveness of the conventional vaccines and medicaments due to virus variation, and is a porcine hemagglutinating encepha lomyelitis virus resistant medicament with a brand-new mechanism.