The invention provides a method for removing micro-glyphosate in glyphosate mother liquor by means of oxidation, which comprises the steps of: adding pH adjusting agent into the glyphosate-containing mother liquor to adjust the pH value of the mother liquor at 0.1-14; raising temperature to a certain temperature; under the protection conditions of temperature preserving, stirring, and adding nitrogen or oxygen, slowly adding strong oxidizing agent and reacting for a certain time by means of reflux; and preserving the temperature at a certain time after adding to lead the oxidizing agent left in the mother liquor to be completely reacted to obtain the mother liquor which contains the glyphosate below 10 PPm. The pH adjusting agent is the combination of one or several materials of hydrochloric acid, vitriol, phosphoric acid, sodium hydroxide, potassium hydroxide and calcium oxide. The strong oxidizing agent is hydrogen peroxide, chloric acid, hypochlorous acid and compound of salts thereof. The method can effectively remove the micro-glyphosate in the complex glyphosate mother liquor, so that the sodium chloride salt removed from the mother liquor with the other methods is used for sweetener or beneficial components after converting the organic phosphorus and nitrogen-containing compound into single phosphate radical and amine salt inorganic compound are used for agricultural fertilizer, and the like; can not generate phytotoxicity; reduces the environment pollution and the soil hardening caused by the mother liquor; and achieves the aim of 'recycling waste material'.