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发明授权
CN101565465B 一种抗刚地弓形虫表面抗原1人源抗体Fab片段及其编码基因 失效 - 权利终止

一种抗刚地弓形虫表面抗原1人源抗体Fab片段及其编码基因
摘要：
本发明属生物技术领域，涉及一种抗刚地弓形虫表面抗原1(SAG1)人源抗体Fab片段及其编码基因和用途。本发明通过构建抗刚地弓形虫人源免疫球蛋白基因文库并经ELISA、稀释凝血酶原时间、测序分析等从库中筛选到抗刚地弓形虫SAG1人源抗体Fab片段，经表达纯化及鉴定，证实所述的人源抗体Fab片段能够特异性识别弓形虫速殖子重组SAG1并与之具有较高的亲和力，与弓形虫速殖子特异性识别。本发明的人源抗体Fab片段，无Fc段，在发挥抑制弓形虫入侵宿主细胞作用的同时，不会激活补体和引起人体免疫反应等病理损害，应用于人体安全可靠。可制备防治弓形虫病的抗体药物或抗体靶向药物。
摘要（英）：

The present invention belongs to the field of biotechnology, and relates to a surface antigen 1 (SAG1) of Toxoplasma gondii human antibody Fab fragment, encoded gene and use thereof. According to the invention, the surface antigen 1 (SAG1) of Toxoplasma gondii human antibody Fab fragment is filtered from a base through establishing a Toxoplasma gondii human immunoglobulin, ELISA, diluting the prothrombin time, sequencing analysis, etc. Through expression purifying and authenticating, the human antigen Fab fragment is authenticated to specifically identify the tachyzoite-bradyzoite recombination SAG1 of Toxoplasma gondii and have higher affinity with the tachyzoite-bradyzoite recombination SAG1 of Toxoplasma gondii, for being identified with the specificity of Toxoplasma gondii tachyzoite-bradyzoite. The human antigen Fab fragment of the invention does not contain Fc segment and does not activate the alexin or cause the histopathological damages of human immune response, etc. when the function of restricting the invasion of Toxoplasma gondii to the host cell is exerted. The surface antigen 1 (SAG1) of Toxoplasma gondii human antibody Fab fragment is safe and reliable when applied for the human body. The antigen medicine for treating toxoplasmosis or the antigen targeted medicine can be prepared.

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