The invention discloses a vipera russelli venoms x coagulation factor activating enzyme, the method to extract and the application of the vipera russelli venoms x coagulation factor activating enzyme.The invention aims to provide an x coagulation factor activating enzyme which originates from vipera russelli venoms and the extraction method and the application in preparing haemostatic and medicine to treat hemorrhage diseases. The enzyme is protein with the activity of x coagulation factor activating enzyme and is obtained in this way that the vipera russelli venoms undergoes exchange chromatography and dialysis of DEAE-SephadexA-50, ion exchange chromatography of cellulose DEAE-32 and the filtration chromatography of gel Superose-12. The X coagulation factor activating enzyme is an active ingredient in preparing the medicines for surgical bleeding, medical hemorrhage, obstetrical bleeding, ophthalmology and otorhinolaryngology hemorrhage, pediatric hemorrhage, the hemorrhage after processing tissue biopsy, the clinic hemorrhage etc and hemorrhage diseases. The invention has a broad prospect and will play an important part in bio-pharmacy field.