The present invention is one kind of 175 kD recombinant protein (PfEBA175-IIF2) of plasmodium falciparum erythrocyte conjugated antigen functional region, its coding DNA sequence, vector containing the DNA sequence, host cell containing the vector, gene engineering process of preparing the recombinant protein, and the application of the recombinant protein in preparing malaria resisting vaccine and performing preclinical research on plasmodium invasion mechanism. The recombinant protein PfEBA175-IIF2 possesses excellent immunogenicity and good bionomics as well as the function of combining with sialic acid dependent erythrocyte, and can initiate effective plasmodium resisting immune response in immunized individuals.