公开(公告)号：US06245548B1

公开(公告)日：2001-06-12

申请号：US09522010

申请日：2000-03-09

Applicant: Annemarie H. Ralston ,  William N. Drohan

Inventor： Annemarie H. Ralston ,  William N. Drohan

IPC: C12N974

CPC classification number: C12N9/6429 ,  A61K38/00 ,  C12Y304/21005

Abstract: A method for the production of thrombin from pure prothrombin is provided by converting pure prothrombin by treating pure prothrombin with sodium citrate in the absence of additional coagulation factors. Preferrably, a solution is used containing about 30% to about 40% sodium citrate.

Abstract translation: 通过在没有额外的凝血因子的情况下通过用纯柠檬酸钠处理纯凝血酶原来转化纯凝血酶原来提供从纯凝血酶原产生凝血酶的方法。 优选地，使用含有约30％至约40％柠檬酸钠的溶液。

公开(公告)号：US06168938A

公开(公告)日：2001-01-02

申请号：US09000228

申请日：1998-01-23

Applicant: Adrian Malcolm Oates ,  Malgorzata Kupczyk ,  Jerry Kanellos

Inventor： Adrian Malcolm Oates ,  Malgorzata Kupczyk ,  Jerry Kanellos

IPC: C12N974

CPC classification number: C12N9/6429 ,  C12Y304/21005

Abstract: A method for the preparation of thrombin from a prothrombin-containing material is characterised in that the prothrombin-containing material is an activated prothrombin complex concentrate ActPCC fraction, which is contacted with divalent cations to convert the prothrombin to thrombin.

Abstract translation: 用于从含凝血酶原的材料制备凝血酶的方法的特征在于，所述含凝血酶原的物质是活化的凝血酶原复合物浓缩物ActPCC级分，其与二价阳离子接触以将凝血酶原转化为凝血酶。

公开(公告)号：US06395880B1

公开(公告)日：2002-05-28

申请号：US09509052

申请日：2000-05-26

Applicant: Yendra Linnau ,  Ernst Hetzl ,  H. Peter Matthiessen ,  Silvia Neppl ,  Wolfgang Schönhofer ,  Hans-Peter Schwarz

Inventor： Yendra Linnau ,  Ernst Hetzl ,  H. Peter Matthiessen ,  Silvia Neppl ,  Wolfgang Schönhofer ,  Hans-Peter Schwarz

IPC: C12N974

CPC classification number: C07K14/8128 ,  A61K38/00

Abstract: A method of purifying antithrombin III (AT III) from a starting material containing an AT III/heparin complex or an AT III/heparinoid complex is disclosed. First, the method comprises adsorbing the AT III/heparin complex or the AT III/heparinoid complex on an anion exchanger material. Second, the method involves separating the AT III from the adsorbed AT III/heparin complex or an AT III/heparinoid complex by elution with a buffer having a pH ranging from 8.5 to 10.5 and a conductivity between 10 and 60 mS.

Abstract translation: 公开了从含有AT III /肝素复合物或AT III /类肝素复合物的原料中纯化抗凝血酶III（AT III）的方法。 首先，该方法包括在阴离子交换材料上吸附AT III /肝素复合物或AT III /类肝素复合物。 第二，该方法包括通过用pH为8.5至10.5和10至60mS的电导率的缓冲液洗脱来分离AT III与吸附的AT III /肝素复合物或AT III /类肝素复合物。