公开(公告)号：US20170275684A1

公开(公告)日：2017-09-28

申请号：US15508861

申请日：2015-09-04

申请人： Zhi ZHENG

发明人： Zhi ZHENG

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6853 ,  C12Q1/6834 ,  C12Q2565/519

摘要： The present application provides simple, specific and sensitive methods for detecting a target nucleic acid in a biological sample, detecting a pathogen in a biological sample, and diagnosing a disease in an individual, utilizing a combination of nucleic acid hybridization-based capture and ligation-enabled PCR. The methods are particularly useful for detecting low level nucleic acids and pathogens and for automation and processing of multiple biological samples.