公开(公告)号：US12203079B2

公开(公告)日：2025-01-21

申请号：US17811764

申请日：2022-07-11

Applicant: ZHEJIANG UNIVERSITY

Inventor： Liuhui Kuang ,  Qi Li ,  Dezhi Wu ,  Guoping Zhang

IPC: C12N15/82 ,  A01H1/04 ,  A01H4/00 ,  A01H6/46

Abstract: An Agrobacterium-mediated genetic transformation method for sea barleygrass is provided. The method includes: S1, selecting immature embryo materials of sea barleygrass with immature embryos each having a length in a range of 0.5-1.0, sterilizing them with alcohol and sodium hypochlorite to obtain sterilized seeds; S2, separating the immature embryos, crosscutting the immature embryos, and inducing callus generation and proliferation; S3, adjusting pre-culture time and Agrobacterium infection time of calli based on the callus generation and Agrobacterium growth to thereby prevent excessive Agrobacterium liquid; and S4, performing adventitious bud induction culture and rooting induction culture under a shielding-formed low-light environment to obtain tissue culture plantlets. It relates to a tissue culture method for immature embryos of sea barleygrass with high green spot differentiation and plantlet formation rates, which is not limited by materials. A transformation and regeneration system has high genetic transformation and mutation efficiency.