公开(公告)号：US20240251802A1

公开(公告)日：2024-08-01

申请号：US18014692

申请日：2022-01-27

Applicant: ZHEJIANG UNIVERSITY

Inventor： Weiguo FANG ,  Dan Tang

IPC: A01N63/30 ,  A01N45/02 ,  A01N63/14 ,  A01P7/04 ,  A01P19/00 ,  C12N15/80 ,  C12P15/00

CPC classification number: A01N63/30 ,  A01N45/02 ,  A01N63/14 ,  A01P7/04 ,  A01P19/00 ,  C12N15/80 ,  C12P15/00

Abstract: The present disclosure provides use of sativene, and a recombinant biocontrol fungus and a muscardine cadaver and use thereof, belonging to the technical field of genetic engineering. The present disclosure provides use of sativene in insect attraction and/or pest control. The sativene are attractive to insects. A higher concentration of the sativene has a stronger attraction on mosquito and Drosophila larvae; the Drosophila larvae respond to the sativene even at a level as low as 10−11 g. The sativene also be attractive to Galleria mellonella larvae. The present disclosure further provides a recombinant biocontrol fungus, including a recombinant fungal expression plasmid, where the recombinant fungal expression plasmid is inserted with a pine longifolene synthesis gene. In the present disclosure, the recombinant biocontrol fungus has increased synthetic amounts and volatilized amounts of the longifolene and sativene, resulting in an enhanced attraction to insects.