公开(公告)号：US20200293934A1

公开(公告)日：2020-09-17

申请号：US16818188

申请日：2020-03-13

申请人： Yunong JI ,  Hirotaka UNNO ,  Shigeo HATADA ,  Manabu SEO

发明人： Yunong JI ,  Hirotaka UNNO ,  Shigeo HATADA ,  Manabu SEO

IPC分类号： G06N7/00

摘要： Provided is a technique capable of adequately presenting the product specifications of a material sample even when the concentration distribution of the material is asymmetrical. A material sample according to the present disclosure stores a material having variation according to a probability distribution. As the product specifications of the material sample, the representative value of the probability distribution as well as an interval in which the amount of the material is greater than or equal to a target probability on the probability distribution is displayed.

公开(公告)号：US20230295702A1

公开(公告)日：2023-09-21

申请号：US18147149

申请日：2022-12-28

申请人： Yuuki Yonekawa ,  Hirotaka Unno ,  Satoshi Nakazawa ,  Yunong Ji ,  Kazusa DNA Research Institute

发明人： Yuuki YONEKAWA ,  Hirotaka UNNO ,  Satoshi NAKAZAWA ,  Yunong JI ,  Sakae ITOGA

IPC分类号： C12Q1/6851 ,  C12Q1/6844

CPC分类号： C12Q1/6851 ,  C12Q1/6844

摘要： A method of determining a limit of detection and a limit of quantitation in a nucleic acid detection test is provided, the method including, by using a container having a specific configuration, adding a negative specimen that does not contain target nucleic acids to a positive control group and adding a reagent used for a nucleic acid detection test to a negative control group and the positive control group to amplify the target nucleic acids, and determining a smallest specific copy number among specific copy numbers with a detection rate of 95% or greater in the positive control group as a limit of detection and determining a smallest specific copy number among specific copy numbers with CVln of 35% or less as a limit of quantitation in a case where the target nucleic acids are not detected in the negative control group.

公开(公告)号：US20230168263A1

公开(公告)日：2023-06-01

申请号：US18159987

申请日：2023-01-26

申请人： Hirotaka UNNO ,  Satoshi NAKAZAWA ,  Yuuki YONEKAWA ,  Yunong JI ,  Yusuke OSAKI

发明人： Hirotaka UNNO ,  Satoshi NAKAZAWA ,  Yuuki YONEKAWA ,  Yunong JI ,  Yusuke OSAKI

IPC分类号： G01N35/00

CPC分类号： G01N35/00623 ,  G01N2035/00653

摘要： An information provision device includes an acquisition unit and an information output unit. The acquisition unit acquires test result data indicating a Cq value of a well obtained by a test device performing a polymerase chain reaction (PCR) test on a standard device having the well in which a known number of copies of a nucleic acid are disposed. The information output unit outputs comparison information obtained by comparing information obtained from evaluation target data that is the evaluation target data of an evaluation target with information obtained from one or more pieces of the test result data different from the evaluation target data.

公开(公告)号：US20210010072A1

公开(公告)日：2021-01-14

申请号：US16766125

申请日：2018-11-22

申请人： Koei SUZUKI ,  Hirotaka UNNO ,  Manabu SEO ,  Satoshi IZUMI ,  Yusuke OSAKI ,  Yudai KAWASHIMA ,  Michie HASHIMOTO

发明人： Koei SUZUKI ,  Hirotaka UNNO ,  Manabu SEO ,  Satoshi IZUMI ,  Yusuke OSAKI ,  Yudai KAWASHIMA ,  Michie HASHIMOTO

IPC分类号： C12Q1/6851 ,  C12Q1/686

摘要： Provided is a device including: a plurality of wells; a reagent composition located in each of the wells and containing an amplifiable reagent in a specific copy number; and two or more groups into which the wells are divided to have the amplifiable reagent located in the same specific copy number but to be varied in composition of the reagent composition except for the specific copy number. Preferably, composition except for the specific copy number of the amplifiable reagent includes at least any one of primer and amplifying reagent. More preferably, the device includes two or more groups varied in the specific copy number. Yet more preferably, the groups of wells include at least a negative control group in which the specific copy number of the amplifiable reagent is 0, and a group in which the specific copy number of the amplifiable reagent is close to the limit of detection.

公开(公告)号：US20210147906A1

公开(公告)日：2021-05-20

申请号：US16766117

申请日：2018-11-22

申请人： Yusuke OSAKI ,  Satoshi IZUMI ,  Yudai KAWASHIMA ,  Michie HASHIMOTO ,  Manabu SEO ,  Koei SUZUKI ,  Hirotaka UNNO

发明人： Yusuke OSAKI ,  Satoshi IZUMI ,  Yudai KAWASHIMA ,  Michie HASHIMOTO ,  Manabu SEO ,  Koei SUZUKI ,  Hirotaka UNNO

IPC分类号： C12Q1/6806 ,  B01L3/02 ,  C12Q1/689 ,  C12Q1/70

摘要： Provided is a detection determining method used in detection of a testing target in a sample by amplification of the testing target and an amplifiable reagent, wherein the amplifiable reagent is provided in a number of 200 or less, the detection determining method including a determining step of determining that the testing target is present and a detection result is positive when the amplifiable reagent is amplified and the testing target is amplified, and determining that the testing target is absent or at least less than a specific copy number of the amplifiable reagent and a detection result is negative when the amplifiable reagent is amplified and the testing target is not amplified. Also provided are a detection determining device, a detection determining program, and a device used for the detection determining method.

公开(公告)号：US20240155769A1

公开(公告)日：2024-05-09

申请号：US18497373

申请日：2023-10-30

申请人： Yukihiro WAKABAYASHI ,  Takashi FUJITA ,  Hiroyuki HIRATSUKA ,  Kohji TAKEUCHI ,  Tohru HASEGAWA ,  Hirotaka UNNO

发明人： Yukihiro WAKABAYASHI ,  Takashi FUJITA ,  Hiroyuki HIRATSUKA ,  Kohji TAKEUCHI ,  Tohru HASEGAWA ,  Hirotaka UNNO

IPC分类号： H05K1/18 ,  H05K1/02

CPC分类号： H05K1/181 ,  H05K1/0287 ,  H05K2201/09972

摘要： A method for forming a functional layer includes giving information to the functional layer while forming the functional layer on a surface of an object. The functional layer includes an information area having the information and a non-information area other than the information area. The information area is different in color from the non-information area.

公开(公告)号：US20220170076A1

公开(公告)日：2022-06-02

申请号：US17455971

申请日：2021-11-22

申请人： Hirotaka Unno ,  Satoshi Nakazawa ,  Yuuki Yonekawa ,  Michie Hashimoto ,  Fasmac Co., Ltd.

发明人： Hirotaka UNNO ,  Satoshi Nakazawa ,  Yuuki Yonekawa ,  Michie Hashimoto ,  Satoshi Futo ,  Mari Onishi ,  Riztyan

IPC分类号： C12Q1/6806 ,  G01N21/64

摘要： A method for manufacturing a device is provided, in which a known quantity of a reagent is reliably immobilized in a reaction field, which can be stored at room temperature, and with which the performance of a real-time PCR apparatus can be correctly evaluated. The method is a method for manufacturing a device having at least one well, in which a specific number of copies of a nucleic acid in the at least one well are immobilized in a reaction field. The method includes a nucleic acid extraction step of extracting the nucleic acid with an enzyme and a drying deactivation step of deactivating the enzyme by drying at 5° C. to 45° C.