公开(公告)号：US09063154B2

公开(公告)日：2015-06-23

申请号：US13871697

申请日：2013-04-26

申请人： XRpro Sciences, Inc.

发明人： Benjamin P. Warner ,  Rebecca L. E. Miller ,  Lori J. Peterson ,  Jennifer A. Berger ,  Eva R. Birnbaum

IPC分类号： C12Q1/02 ,  G01N21/76 ,  G01N23/223 ,  G01N33/50 ,  G01N33/68

CPC分类号： G01N33/6872 ,  G01N23/2076 ,  G01N23/223 ,  G01N33/502 ,  G01N2223/076

摘要： The present invention comprises a method for measuring the transport of analytes through a cell. The method comprises the steps of providing one or more cells containing at least 10 picograms of an analyte within a volume defined by the area of an x-ray excitation beam and a depth of live times the 1/e attenuation, depth for at least one characteristic x-ray signal of the analyte as attenuated by water. The method further comprises the step of removing at least a of portion of the analyte from the cells. The method also comprises the steps of exciting at least a portion of the analyte that has been removed from the cells with an x-ray beam; and using an x-ray detector to measure x-ray fluorescence.

摘要翻译： 本发明包括用于测量分析物通过细胞的转运的方法。 该方法包括以下步骤：提供一个或多个细胞，其在由x射线激发束的区域限定的体积内的至少10皮克分析物和1 / e衰减深度的至少一个 分析物的特征X射线信号被水减弱。 该方法还包括从细胞中去除至少一部分分析物的步骤。 该方法还包括以x射线束激发已经从细胞中除去的分析物的至少一部分的步骤; 并使用X射线检测器来测量x射线荧光。