公开(公告)号：US20240317882A1

公开(公告)日：2024-09-26

申请号：US18557875

申请日：2021-11-19

申请人： WUHAN BIO-RAID BIOTECH CO., LTD

发明人： Tongcun ZHANG ,  Haichuan ZHU ,  Jiangzhou SHI

IPC分类号： C07K16/28 ,  A61K39/00 ,  A61P35/00 ,  A61P35/02 ,  C07K14/54 ,  C07K14/705 ,  C07K14/725 ,  C12N15/86

CPC分类号： C07K16/2896 ,  A61K39/4611 ,  A61K39/4631 ,  A61K39/4635 ,  A61K39/464429 ,  A61P35/00 ,  A61P35/02 ,  C07K14/5418 ,  C07K14/7051 ,  C07K14/70517 ,  C07K14/70521 ,  C07K14/70578 ,  C12N15/86 ,  A61K2239/15 ,  A61K2239/17 ,  A61K2239/21 ,  A61K2239/22 ,  C07K2317/53 ,  C07K2317/622 ,  C07K2319/03 ,  C07K2319/22 ,  C12N2740/15043

摘要： Disclosed herein is an optimized CD99-targeting chimeric antigen receptor and application thereof, wherein a signal peptide, a single-chain antibody ScFv, strepII, a CD8 hinge, a CD28 transmembrane region, a CD28 intracellular domain, an intracellular co-stimulatory domain 4-1BB and CD3ζ chain are sequentially spliced in the chimeric antigen receptor is from the N-terminal to the C-terminal. The single-chain antibody ScFv can specifically recognize CD99 protein on the surface of tumor cells. The CD99-targeting chimeric antigen receptor is used to modify immune cells for the treatment of surface CD99-positive tumors.