公开(公告)号：US20200231956A1

公开(公告)日：2020-07-23

申请号：US16650230

申请日：2018-09-28

Applicant: VIB VZW ,  UNIVERSITEIT GENT

Inventor： Nico Callewaert ,  Sven Eyckerman ,  Simon Devos

IPC: C12N9/48 ,  C12N9/64 ,  G01N33/68

Abstract: The present invention relates to the field of biochemistry, more particularly to proteomics, more particularly to protein sequencing, even more particularly to single molecule peptide sequencing. The invention discloses means and methods for single molecule protein sequencing and/or amino acid identification using cleavage inducing agent. Said cleavage inducing agents which are not specific for one particular amino acid, cleave polypeptides step by step from the N-terminus onwards and provide information on the identity of the cleaved amino acids based on the kinetics of said reaction.

公开(公告)号：US12019076B2

公开(公告)日：2024-06-25

申请号：US17817908

申请日：2022-08-05

Applicant: VIB VZW ,  Universiteit Gent

Inventor： Nico Callewaert ,  Sven Eyckerman ,  Simon Devos

IPC: G01N33/68 ,  C12N9/48 ,  C12N9/64

CPC classification number: G01N33/6812 ,  C12N9/485 ,  C12N9/641 ,  G01N33/68 ,  G01N33/6818 ,  G01N33/6824

Abstract: The present invention relates to the field of biochemistry, more particularly to proteomics, more particularly to protein sequencing, even more particularly to single molecule peptide sequencing. The invention discloses methods for single molecule protein sequencing and/or amino acid identification using cleavage inducing agents which are not specific for one particular amino acid, cleave polypeptides step by step from the N-terminus onwards and provide information on the identity of the cleaved amino acids based on the kinetics.

公开(公告)号：US20220214354A1

公开(公告)日：2022-07-07

申请号：US17600437

申请日：2020-04-01

Applicant: VIB VZW ,  UNIVERSITEIT GENT

Inventor： Nico Callewaert ,  Simon Devos ,  Sven Eyckerman ,  Joel Vandekerckhove

IPC: G01N33/68

Abstract: The present application relates to the field of protein sequencing, more particularly to protein profiling using massively parallel sequencing with single-molecule sensitivity. Methods, assays and reagents are provided for sequencing individual protein or polypeptide molecules. Also provided are methods and assays for the parallel sequencing of proteins or polypeptides. To this end, particular labeled probes are used that are reactive with the N-terminal amino acid of the polypeptide molecules and can be detected while still associated with the polypeptide(s).

公开(公告)号：US20230221329A1

公开(公告)日：2023-07-13

申请号：US17817908

申请日：2022-08-05

Applicant: VIB VZW ,  Universiteit Gent

Inventor： Nico Callewaert ,  Sven Eyckerman ,  Simon Devos

IPC: G01N33/68 ,  C12N9/48 ,  C12N9/64

CPC classification number: G01N33/6812 ,  C12N9/485 ,  C12N9/641 ,  G01N33/6824 ,  G01N33/6818 ,  G01N33/68

Abstract: The present invention relates to the field of biochemistry, more particularly to proteomics, more particularly to protein sequencing, even more particularly to single molecule peptide sequencing. The invention discloses means and methods for single molecule protein sequencing and/or amino acid identification using cleavage inducing agent. Said cleavage inducing agents which are not specific for one particular amino acid, cleave polypeptides step by step from the N-terminus onwards and provide information on the identity of the cleaved amino acids based on the kinetics of said reaction.

公开(公告)号：US11573238B2

公开(公告)日：2023-02-07

申请号：US16650230

申请日：2018-09-28

Applicant: VIB VZW ,  UNIVERSITEIT GENT

Inventor： Nico Callewaert ,  Sven Eyckerman ,  Simon Devos

IPC: G01N33/68 ,  C12N9/48 ,  C12N9/64

Abstract: The present invention relates to the field of biochemistry, more particularly to proteomics, more particularly to protein sequencing, even more particularly to single molecule peptide sequencing. The invention discloses methods for single molecule protein sequencing and/or amino acid identification using cleavage inducing agents which are not specific for one particular amino acid, cleave polypeptides step by step from the N-terminus onwards and provide information on the identity of the cleaved amino acids based on the reaction kinetics.

公开(公告)号：US20230021352A1

公开(公告)日：2023-01-26

申请号：US17783595

申请日：2020-12-09

Applicant: VIB VZW ,  UNIVERSITEIT GENT

Inventor： Nico Callewaert ,  Simon Devos ,  Sven Eyckerman

IPC: C12Q1/37 ,  C12N9/52 ,  G01N33/68

Abstract: The present invention relates to protein sequencing, more particularly the invention discloses improved aminopeptidases for single molecule protein sequencing and/or amino acid identification. Said aminopeptidases can enzymatically cleave off N-terminal amino acids and are highly suitable in a kinetics-based peptide sequencing approach. Based on the kinetics of the cleaving reaction or of the engagement between said aminopeptidases and peptide to be sequenced, information on the identity of the cleaved amino acids is provided.