公开(公告)号：US20240092879A1

公开(公告)日：2024-03-21

申请号：US17937666

申请日：2022-10-03

Applicant: UNIVERSIDAD NACIONAL AUTONOMA DE MEXICO

Inventor： Lidia RIAÑO UMBARILA ,  José Alberto ROMERO MORENO ,  Lourival Domingos POSSANI POSTAY ,  Baltazar BECERRIL LUJÁN

IPC: C07K16/18 ,  A61P39/00

CPC classification number: C07K16/18 ,  A61P39/00 ,  A61K2039/505 ,  C07K2317/21 ,  C07K2317/55 ,  C07K2317/622

Abstract: The present invention relates to a new composition of human recombinant antibody fragments in both single chain or Fab format, which is capable of completely neutralizing the venom of the scorpion Centruroides sculpturatus Ewing (C. sculpturatus. In particular the new composition comprises the scFv fragment 10FG2 (SEQ. ID. No: 1), which has broad cross-reactivity against various Mexican scorpion venom toxins, and the scFv fragment LR (SEQ. ID. No: 2), with more limited cross-reactivity. Alternatively, the new composition comprises the Fab 10FG2 fragment (SEQ. ID. No: 3 and SEQ.ID. No:4) and the Fab LR fragment (SEQ. ID. No: 5 and SEQ.ID. No 6). The two antibody fragments recognize independent epitopes, present in the two main toxins of the scorpion C. sculpturatus, so that they do not interfere with each other during their binding to the same, on the contrary the antibody fragments of the present invention complement the neutralizing activity.

公开(公告)号：US20210214303A1

公开(公告)日：2021-07-15

申请号：US17058962

申请日：2019-05-20

Applicant: The Board of Trustees of the Leland Stanford Junior University ,  UNIVERSIDAD NACIONAL AUTONOMA DE MEXICO ,  INSTITUTO NACIONAL DE CIENCIAS MEDICAS Y NUTRICION SALVADOR ZUBIRAN

Inventor： Richard Neil ZARE ,  Jose Ignacio VEYTIA-BUCHELI ,  Edson Norberto Carcamo NORIEGA ,  Gnanamani ELUMALAI ,  Shyam SATHYAMOORTHI ,  Lourival Domingos POSSANI POSTAY ,  Shibdas BANERJEE ,  Rogelio Enrique HERNANDEZ PANDO

IPC: C07C323/22 ,  C07C319/20 ,  A61P35/02 ,  A61P31/04

Abstract: Provided are colored 1,4-benzoquinone compounds obtained by oxidation of precursor molecules from the venom of the scorpion Diplocentrus melici (Diplocentridae family). Schemes for the chemical synthesis of these compounds using reagents commercially available are also provided. Biological assays show that the red compound (3,5-dimethoxy-2-(methylthio)cyclohexa-2,5-diene-1,4-dione) is very effective at killing Staphylococcus aureus and that the blue compound (5-methoxy-2,3-bis(methylthio)cyclohexa-2,5-diene-1,4-dione) has remarkable activity against Mycobacterium tuberculosis. The blue compound is effective against multi-drug-resistant tuberculosis (MDR-TB) and is not detrimental to lung epithelium. Both compounds were found to be cytotoxic to human neoplastic cell lines and to mononuclear cells (PBMCs).