公开(公告)号：US20220389407A1

公开(公告)日：2022-12-08

申请号：US17827240

申请日：2022-05-27

申请人： The Trustees of Boston College

发明人： Tim van Opijnen ,  Juan C. Ortiz-Marquez ,  Stephen Hummel ,  José Bento

IPC分类号： C12N15/10 ,  C12Q1/6811

摘要： The present disclosure provides a novel approach, recomSELEX, that highly integrate mutually supportive recombination and computational methods for aptamer selection and identification. The recomSELEX approach comprises a recombinatorial SELEX platform for aptamer screening that exponentially increases the sequence space that is explored by incorporation of a DNA shuffling step that allows recombination between aptamers. Subsequently, the recombinatorial SELEX platform can also be employed to develop new and optimize already existing aptamers. The recomSELEX further comprises a computational SELEX platform with a constrained genetic algorithm (GA) to identify potential aptamers that are stable and have the desired specificity and affinity of a target.