公开(公告)号：US20150004713A1

公开(公告)日：2015-01-01

申请号：US14296034

申请日：2014-06-04

Applicant: The Regents of the University of California

Inventor： Na Li ,  Fang Wei ,  Huaping Li ,  Guillermo C. Bazan

IPC: G01N31/22 ,  G01N21/78 ,  G01N21/82

CPC classification number: G01N31/22 ,  B82Y15/00 ,  G01N21/554 ,  G01N21/78 ,  G01N21/82 ,  Y10S977/773 ,  Y10S977/81 ,  Y10S977/921 ,  Y10T436/147777

Abstract: Methods and materials for the detection of melamine in test samples such as foodstuffs are described. Embodiments of the invention comprise combining a test sample suspected of containing melamine with a particle that produces a colorimetric and/or turbidimetric signal that is dependent upon the concentration of melamine in the test sample. In certain embodiments of the invention, the particles and test sample are combined together with a chemical compound selected to induce the aggregation melamine in a manner that amplifies the colorimetric and/or turbidimetric signal of the assay. In some embodiments of the invention, the aggregation inducing agent is not physically coupled to the particles used in the assays. In other embodiments of the invention, the aggregation inducing agent is physically coupled to the particles used in these assays.

Abstract translation: 描述了测试样品如食品中检测三聚氰胺的方法和材料。 本发明的实施方案包括将怀疑含有三聚氰胺的测试样品与产生取决于测试样品中三聚氰胺浓度的比色和/或比浊信号的颗粒组合。 在本发明的某些实施方案中，颗粒和测试样品与选择用于以扩增测定的比色和/或比浊信号的方式诱导聚集三聚氰胺的化学化合物组合在一起。 在本发明的一些实施方案中，聚集诱导剂不与用于测定中的颗粒物理偶联。 在本发明的其它实施方案中，聚集诱导剂与这些测定中使用的颗粒物理耦合。