公开(公告)号：US20140048417A1

公开(公告)日：2014-02-20

申请号：US14063884

申请日：2013-10-25

申请人： The Regents of the University of California

发明人： Michael Heller ,  Benjamin Sullivan ,  Rajaram Krishnan ,  Dennis Carson ,  Sadik C. Esener

IPC分类号： B03C5/00

CPC分类号： B03C5/005 ,  G01N27/447 ,  G01N30/0005 ,  G01N33/491

摘要： Devices and techniques are described that involve a combination of multidimensional electrokinetic, dielectrophoretic, electrophoretic and fluidic forces and effects for separating cells, nanovesicles, nanoparticulates and biomarkers (DNA, RNA, antibodies, proteins) in high conductance (ionic) strength biological samples and buffers. In disclosed embodiments, a combination of continuous and/or pulsed dielectrophoretic (DEP) forces, continuous and/or pulsed field DC electrophoretic forces, microelectrophoresis and controlled fluidics are utilized with arrays of electrodes. In particular, the use of chambered DEP devices and of a properly scaled relatively larger electrode array devices that combines fluid, electrophoretic and DEP forces enables both larger and/or clinically relevant volumes of blood, serum, plasma or other samples to be more directly, rapidly and efficiently analyzed. The invention enables the creation of “seamless” sample-to-answer diagnostic systems and devices. The devices and techniques described can also carry out the assisted self-assembly of molecules, polymers, nanocomponents and mesoscale entities into three dimensional higher order structures.

摘要翻译： 描述了涉及多维电动，介电泳，电泳和流体力的组合的装置和技术，以及用于在高电导（离子）强度生物样品和缓冲液中分离细胞，纳米囊，纳米颗粒和生物标志物（DNA，RNA，抗体，蛋白质）的作用 。 在所公开的实施例中，连续和/或脉冲介电电泳（DEP）力，连续和/或脉冲场DC电泳力，微电泳和受控流体学的组合被利用于电极阵列。 特别地，使用腔室DEP装置和结合流体，电泳和DEP力的适当缩放的相对较大的电极阵列装置的使用使得血液，血清，血浆或其它样品的较大和/或临床相关体积能够更直接地， 快速有效地分析。 本发明能够创建“无缝”的样本到应答诊断系统和设备。 所描述的装置和技术还可以将分子，聚合物，纳米组分和中尺度实体的辅助自组装执行成三维高阶结构。

公开(公告)号：US20210380546A1

公开(公告)日：2021-12-09

申请号：US17287914

申请日：2019-10-22

申请人： The Regents of the University of California

发明人： Dennis Carson ,  Mary Patricia Corr ,  Howard B. Cottam ,  Tomoko Hayashi ,  Nikunj Shukla

IPC分类号： C07D277/44 ,  A61K39/39

摘要： Compounds useful as an adjuvant, e.g., formulas (I)-(VI) and uses thereof, for example, with immunogenic moieties or other adjuvants, are provided.

公开(公告)号：US10766962B2

公开(公告)日：2020-09-08

申请号：US15736646

申请日：2016-06-16

申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

发明人： Karl Willert ,  Dennis Carson

IPC分类号： C07K16/00 ,  C07K16/28 ,  C07K16/30

摘要： The present invention is directed to antibodies and fragments thereof having binding specificity for FZD7, which preferably do not substantially interact with (bind to) other FZD family members, and methods of making said anti-FZD7 antibodies and binding fragments thereof. Another embodiment relates to antibodies and binding fragments comprising sequences of VH, VL and/or CDR polypeptides described herein. The invention also contemplates conjugates of anti-FZD7 antibodies and binding fragments. The invention further contemplates use of anti-FZD7 antibodies and binding fragments for tissue engineering. This invention also relates to a transgenic mouse expressing the epitope of anti-FZD7 antibodies and binding fragments thereof. Embodiments also pertain to use of anti-FZD7 antibodies and binding fragments (as well as the identified FZD7 epitope) for diagnosis, assessment, prevention and/or treatment of diseases and disorders associated with aberrant FZD7 expression, such as cancer. The invention further provides peptides for use in anticancer vaccines and for producing antibodies.

公开(公告)号：US12091393B2

公开(公告)日：2024-09-17

申请号：US17287914

申请日：2019-10-22

申请人： The Regents of the University of California

发明人： Dennis Carson ,  Mary Patricia Corr ,  Howard B. Cottam ,  Tomoko Hayashi ,  Nikunj Shukla

IPC分类号： C07D277/44 ,  A61K39/39 ,  A61K39/00

CPC分类号： C07D277/44 ,  A61K39/39 ,  A61K2039/55511

摘要： Compounds of formulae (I)-(VD):

A1-Q-L1-A2-L2-Z  Formula I

are useful for enhancing or prolonging an immune response, such as, in a vaccine as an adjuvant or a co-adjuvant.

公开(公告)号：US08932447B2

公开(公告)日：2015-01-13

申请号：US14063884

申请日：2013-10-25

申请人： The Regents of the University of California

发明人： Michael Heller ,  Benjamin Sullivan ,  Rajaram Krishnan ,  Dennis Carson ,  Sadik C. Esener

IPC分类号： B03C5/02 ,  B03C5/00 ,  G01N27/447 ,  G01N33/49 ,  G01N30/00

CPC分类号： B03C5/005 ,  G01N27/447 ,  G01N30/0005 ,  G01N33/491

摘要： Devices and techniques are described that involve a combination of multidimensional electrokinetic, dielectrophoretic, electrophoretic and fluidic forces and effects for separating cells, nanovesicles, nanoparticulates and biomarkers (DNA, RNA, antibodies, proteins) in high conductance (ionic) strength biological samples and buffers. In disclosed embodiments, a combination of continuous and/or pulsed dielectrophoretic (DEP) forces, continuous and/or pulsed field DC electrophoretic forces, microelectrophoresis and controlled fluidics are utilized with arrays of electrodes. In particular, the use of chambered DEP devices and of a properly scaled relatively larger electrode array devices that combines fluid, electrophoretic and DEP forces enables both larger and/or clinically relevant volumes of blood, serum, plasma or other samples to be more directly, rapidly and efficiently analyzed. The invention enables the creation of “seamless” sample-to-answer diagnostic systems and devices. The devices and techniques described can also carry out the assisted self-assembly of molecules, polymers, nanocomponents and mesoscale entities into three dimensional higher order structures.

摘要翻译： 描述了涉及多维电动，介电泳，电泳和流体力的组合的装置和技术，以及用于在高电导（离子）强度生物样品和缓冲液中分离细胞，纳米囊，纳米颗粒和生物标志物（DNA，RNA，抗体，蛋白质）的作用 。 在所公开的实施例中，连续和/或脉冲介电电泳（DEP）力，连续和/或脉冲场DC电泳力，微电泳和受控流体学的组合被利用于电极阵列。 特别地，使用腔室DEP装置和结合流体，电泳和DEP力的适当缩放的相对较大的电极阵列装置的使用使得血液，血清，血浆或其它样品的较大和/或临床相关体积能够更直接地， 快速有效地分析。 本发明能够创建“无缝”的样本到应答诊断系统和设备。 所描述的装置和技术还可以将分子，聚合物，纳米组分和中尺度实体的辅助自组装执行成三维高阶结构。