公开(公告)号：US20130225515A1

公开(公告)日：2013-08-29

申请号：US13762925

申请日：2013-02-08

Applicant: Temasek Life Sciences Laboratory Limited

Inventor： Rajesh Narhari PATKAR ,  Naweed Isaak NAQVI

IPC: A61K31/7048

CPC classification number: A61K31/7048 ,  C07K1/16 ,  C07K14/37

Abstract: Methods of isolating biologically active molecules from an organism, for example from a fungus, are described. The methods involve isolating an active molecule which possesses a specific biological activity on, in or against wild-type yeast cells, but not on, in or against yeast cells that express a given ABC transporter from a given organism, including but not limited to Magnaporthe species such as Magnaporthe grisea. In preferred embodiments, the biological activity is antifungal activity. In most preferred embodiments, the antifungal activity is against a human pathogen, including but not limited to Candida albicans. The biologically active molecules can be formulated as pharmaceutical compositions and used to treat fungal infections in a subject, including a human subject.

Abstract translation: 描述了从生物体（例如真菌）分离生物活性分子的方法。 所述方法包括分离在野生型酵母细胞中或对抗野生型酵母细胞但不包括表达来自给定生物体的给定ABC转运蛋白的酵母细胞中或之上具有特定生物活性的活性分子，包括但不限于Magnaporthe 物种如Magnaporthe grisea。 在优选的实施方案中，生物活性是抗真菌活性。 在最优选的实施方案中，抗真菌活性是针对人类病原体，包括但不限于白色念珠菌。 生物活性分子可以配制成药物组合物并用于治疗受试者（包括人类受试者）中的真菌感染。